Solution structure of the antiapoptotic protein bcl-2

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Solution structure of the antiapoptotic protein bcl-2.

The structures of two isoforms of Bcl-2 that differ by two amino acids have been determined by NMR spectroscopy. Because wild-type Bcl-2 behaved poorly in solution, the structures were determined by using Bcl-2/Bcl-x(L) chimeras in which part of the putative unstructured loop of Bcl-2 was replaced with a shortened loop from Bcl-x(L). These chimeric proteins have a low pI compared with the wild-...

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Immunohistochemical Demonstration of Antiapoptotic Proliferating Protein Bcl-2 in the Ameloblastomas

Apoptosis, also known as programmed cell death or physiologic cell death, plays diverse roles in embryogenesis and normal homeostasis as well as in oncogenesis. Apoptotic processes are modulated by several geneencoded products, including the Bcl-2 family proteins, which have inhibitory or stimulatory effects.1 Bcl-2 derives its name from B-cell lymphoma 2, as it is the second member of a range ...

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PANCREATIC CANCER Variants of bcl-2 specific siRNA for silencing antiapoptotic bcl-2 in pancreatic cancer

Background and aims: Pancreatic cancer remains a devastating diagnosis with only limited therapeutic options. Specific inhibition of expression of target genes has become possible using small interfering (si) RNAs. We therefore investigated how far siRNA specific for bcl-2 may serve as a therapeutic option for pancreatic cancer in vitro and in vivo. Methods: siRNAs targeting two different regio...

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Variants of bcl-2 specific siRNA for silencing antiapoptotic bcl-2 in pancreatic cancer.

BACKGROUND AND AIMS Pancreatic cancer remains a devastating diagnosis with only limited therapeutic options. Specific inhibition of expression of target genes has become possible using small interfering (si) RNAs. We therefore investigated how far siRNA specific for bcl-2 may serve as a therapeutic option for pancreatic cancer in vitro and in vivo. METHODS siRNAs targeting two different regio...

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The autophagy protein Atg12 associates with antiapoptotic Bcl-2 family members to promote mitochondrial apoptosis.

Autophagy and apoptosis constitute important determinants of cell fate and engage in a complex interplay in both physiological and pathological settings. The molecular basis of this crosstalk is poorly understood and relies, in part, on "dual-function" proteins that operate in both processes. Here, we identify the essential autophagy protein Atg12 as a positive mediator of mitochondrial apoptos...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 2001

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.041619798